Last edited by Bragis
Monday, May 11, 2020 | History

4 edition of Enzymatic probes of lipoprotein structure and function found in the catalog.

Enzymatic probes of lipoprotein structure and function

Nikhil Mohan Pattnaik

Enzymatic probes of lipoprotein structure and function

action of lipolytic enzymes on human serum high density lipoproteins.

by Nikhil Mohan Pattnaik

  • 372 Want to read
  • 27 Currently reading

Published .
Written in English


Classifications
LC ClassificationsMicrofilm 45777
The Physical Object
FormatMicroform
Paginationvi, 84 p.
Number of Pages84
ID Numbers
Open LibraryOL1249641M
LC Control Number94895188

Lipoprotein lipase exists widely in human bodies. It is distributed in adipose, heart, skeletal muscle tissue, as well as in lactating mammary glands. It participates in the metabolism of glycerolipid and it is a rate-limiting enzyme for the hydrolysis of triglyceride (TG)-rich lipoproteins. When a circulating lipoprotein touches a cell, if the cell expresses lipoprotein lipase (LPL), first LPL would trap the lipoprotein cargo, and then will start to catabolize the TG. Importantly, there are no efficient TG cargoes inside the cell, so the function of lipoprotein lipase is to make fat easy to move inside the cell.

Certain variations in the LPL gene have been shown to influence the levels of fats in the bloodstream. The LPL gene variants likely result in the production of lipoprotein lipase enzymes with altered abilities to break down triglycerides. In some cases, the enzyme is overactive, resulting in low fat levels. In other cases, the enzyme is impaired, resulting in increased fat . Very-low-density lipoproteins (VLDL) contain apoprotein B (apo B), are synthesized in the liver, and transport TGs and cholesterol to peripheral tissues. VLDL is the way the liver exports excess TGs derived from plasma free fatty acids and chylomicron remnants; VLDL synthesis increases with increases in intrahepatic FFAs, such as occur with high-fat diets and when .

Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-. Other articles where Lipoprotein envelope is discussed: virus: The lipoprotein envelope: Surrounding viruses of either helical or icosahedral symmetry are lipoprotein envelopes, unit membranes of two lipid layers interspersed with protein molecules (lipoprotein bilayer). These viral membranes are composed of phospholipids and neutral lipids (largely cholesterol) derived .


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Enzymatic probes of lipoprotein structure and function by Nikhil Mohan Pattnaik Download PDF EPUB FB2

Lipoprotein(a) is similar in structure to LDL. However, it contains a additional protein, apolipoprotein(a) (apo-(a)), covalently bound to apo-B.

Apo-(a) has been found to have a high sequence homology with plasminogen. It contains variable amounts of repeating kringle regions and more than 40 isoforms with a MW range of kD. Thus, the two-domain structure of apo E has been shown to lead to two different lipid-bound conformations on lipoprotein-like spherical particles.

The N-terminal four-helix bundle can adopt either open or closed conformations resulting from binding competition with the C-terminal domain that has higher lipid affinity, or from other types of. High density lipoproteins (HDLs) were first identified in the late s and early s as the smallest and densest of all the lipoprotein classes ().For many years, however, most of the attention of researchers in the lipoprotein field focused on the relationship between plasma low density lipoprotein cholesterol levels and the development of cardiovascular disease, with little Cited by: 4.

A lipoprotein is a biochemical assembly whose primary purpose is to transport hydrophobic lipid (also known as fat) molecules in water, as in blood plasma or other extracellular have a single-layer phospholipid and cholesterol outer shell, with the hydrophilic portions oriented outward toward the surrounding water and lipophilic portions of each molecule oriented.

The gathering of data on the function of membrane proteins prior to knowledge of their structure is valuable for characterizing and defining the proteins. Once the structure is known, another stage of research will penetrate to the functional assignments of the structure.

Lipoprotein lipase: structure, function, regulation, and role in disease. Mead JR(1), Irvine SA, Ramji DP. Lipoprotein lipase (LPL) catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins, thereby providing non-esterified fatty acids and 2-monoacylglycerol for tissue Cited by: Lipoprotein structure Ana Jonas Department ~/" Biochemistry, College of Medicine, Universi O' of Illinois at Urhana-Champaign, They undergo enzymatic reactions of their lipid components, facilitated and spontaneous The function of LDL is to deliver cholesteryl esters to peripheral tissues and to the liver.

HDL are synthesized and File Size: 3MB. Lipoprotein lipase (LPL) (EC ) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial is a water-soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol s: LPL, HDLCQ11, LIPD, lipoprotein lipase.

Lipoprotein lipase: Structure, function, regulation, and role in disease Article Literature Review in Journal of Molecular Medicine 80(12) January with Reads. Abstract. In spite of extensive investigations by a variety of physical and chemical techniques, the structural organization of the protein and lipid constituents of the major classes of serum lipoproteins, very low- (VLDL), low- (LDL) and high-density (HDL), remains to be determined (1,2).Author: Angelo M.

Scanu. Lipoprotein, any member of a group of substances containing both lipid (fat) and protein. They occur in both soluble complexes, such as egg yolk, and insoluble ones, such as cell membranes. Lipoproteins in blood plasma have been intensively studied.

Functions of Apo proteins (1) They can form part of the structure of the lipoprotein, e.g. apo B, structural component of VLDL and Chylomicons (2) They are enzyme cofactors, e.g.

C-II for lipoprotein lipase, A-I for lecithin: cholesterol acyl transferase (LCAT), or enzyme inhibitors, eg, apo A-II and apo CIII for lipoprotein lipase, apo C-I for. Lipoproteins are macromolecular structures (supramolecular complexes) formed by the association of proteins and lipids, whose main function is to facilitate the transport of lipids (apolar compounds) in the aqueous environment (polar) that is blood.

We can distinguish in a lipoprotein an envelope and the core. The envelope is formed mainly by amphipatic lipids. lipoprotein structure, composition and kinetics and then relate those parameters to risk.

There are many ways of assaying lipid and lipoprotein concentrations and characteristics: particle numbers, particle sizes, particle lipid content, particle apolipoprotein content, etc.

Lipoprotein lipase (LPL) plays a central role in the hydrolysis of circulating triglycerides present in chylomicrons, and very low density lipoproteins. The active form of the enzyme is a non-covalent homodimer which contains multiple functional domains required for normal hydrolytic activity including a catalytic domain, as well as sites involved in co-factor, Cited by: Structure of a lipoprotein.

Lipoproteins have a single layer of phospholipid molecules on their outside, surrounding a central core. (By contrast, plasma membranes are comprised of a BILAYER.) Since the polar part of each phospholipid faces out, the outside of the phospholipid molecule is polar and thus compatible with the surrounding water.

As a member, you'll also get unlimited access to o lessons in math, English, science, history, and more. Plus, get practice tests, quizzes, and. Nikhil Mohan Pattnaik is an Indian scholar, scientist, and science author.

He obtained a PhD from the University of Chicago in mater: Indian Institute of Technology Kanpur. Please Note: You may not embed one of our images on your web page without a link back to our site.

If you would like a large, unwatermarked image for your web page or blog, please purchase the appropriate license. Enzymatic Assay of LIPOPROTEIN LIPASE (E.C.

) PROCEDURE: Pipette (in milliliters) the following reagents into suitable cuvettes: Test Blank Reagent A (Buffer) Reagent D (Enzyme Solution) Mix by inversion and equilibrate to 37°C. Monitor the A nm until constant, using a suitablyFile Size: 20KB.

Lipoproteins function in maintaining cholesterol homeostasis, transporting the molecule from site to site (synthesis to biochemical action), and lastly to the liver for excretion.

Lipoprotein Structure. Each lipoprotein particle consists of a core of hydrophobic lipids surrounded by a shell of relatively more polar lipids and proteins.The function of LDL in metabolism is mediating by cellular uptake via receptor-mediated endocytosis followed by lysosomal degradation [1,2], and is strongly dependent on the lipid distribution, the structure of LDL particles and on the proper conformational orientation of apolipoprotein B (apo-B).

Apo-B is the sole protein component of Cited by: 4.Diazyme's Lipoprotein (a) Lp(a) is a dual vial, liquid stable reagent system which provides reliable results. The Lp(a) assay features time saving instrument specific packaging options which eliminate reagent transfer and increases operator efficiency.

The assay method requires only 6 μL of serum sample and is completed in less than 10 minutes.